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?MONOSACCHARIDE COMPOSITION OF HIGH MOLECULAR WEIGHT CULTURE FILTRATE ELICITOR OF COLLETOTRICHUM LINDEMUTHIANUM, DETERMINED BY HIGH PERFORMANCE ANION-EXCHANGE/PULSED AMPEROMETRIC DETECTION (HPAE-PAD)

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Title: ?MONOSACCHARIDE COMPOSITION OF HIGH MOLECULAR WEIGHT CULTURE FILTRATE ELICITOR OF COLLETOTRICHUM LINDEMUTHIANUM, DETERMINED BY HIGH PERFORMANCE ANION-EXCHANGE/PULSED AMPEROMETRIC DETECTION (HPAE-PAD)

Authors: Fatima Bi, Paul Finch

Journal: Pakistan Journal of Scientific and Industrial Research (Series B: Biological Sciences)

HEC Recognition History
Category From To
Y 2023-07-01 2024-09-30
Y 2022-07-01 2023-06-30
Y 2021-07-01 2022-06-30
Y 2020-07-01 2021-06-30
Y 1900-01-01 2005-06-30

Publisher: PCSIR Scientific Information Centre

Country: Pakistan

Year: 1998

Volume: 41

Issue: 6

Language: English

Keywords: Colletotrichum lindemuthianum Elicitor Monosaccharide composition.

Categories
Carbohydrate Chemistry (8) Plant Pathology (5535) Microbiology (14542) Biochemistry (18816) Analytical Chemistry (1778)

Abstract

Monosaccharide composition of crude and partially purified high molecular weight culture filtrate elicitor (HMWCFE) fractions-I and II isolated by molecular sieve chromatography from Colletotrichutn lindemuthianum 1M! (112166) isolate were acid hydrolysed for 0.5, 1.5, 3 and 5 h. Liberated monosaccharides were analysed directly using high performance anion-exchange chromatography with pulsed amperometric detection (HPAE-PAD) Mannose galactose were found as major sugars of each sample whereas glucosamine, arabinose and rhamnose were additional sugars. Glucosamine was found as a major sugar (26% w/w) of Fraction-I and first time reported from preparations of this organism.

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