DefinePK hosts the largest index of Pakistani journals, research articles, news headlines, and videos. It also offers chapter-level book search.
Title: Evaluation of Early Growth Response of Wheat Genotypes Grown Under PEG-Mediated Water Stress Conditions
Authors: Muhammad Mohibullah, Wasif Rasool, Nasr Ullah Khan, Sundas Batool, Asif Rasool
Journal: International Journal of Agriculture Innovations and Cutting-Edge (JAI)
| Category | From | To |
|---|---|---|
| Y | 2024-10-01 | 2025-12-31 |
Publisher: Agricultural and Biological Sciences (miscellaneous)
Country: Pakistan
Year: 2024
Volume: 2
Issue: 4
Language: English
Keywords: Durum wheatPEG Drought AZRC Dera Akbar-19 Wheat landrace
The yield of bread wheat (Triticum aestivum L.), an important staple food cereal crop, is constantly challenged by drought stress. The unexpected changes in rainfall and heat spells induced by climate change further aggravate wheat production. Strategies to fast-screen wheat genotypes for drought stress in a controlled environment are key to wheat breeding programs' success in developing drought-tolerant wheat cultivars. The current study was designed to evaluate the early growth response of wheat genotypes grown under PEG-mediated water stress conditions. Six wheat genotypes comprising local wheat cultivars, landraces and durum wheat advanced lines were grown in Petr-dishes and pots under PEG-mediated water stress conditions (5% and 10% PEG), using a Completely Randomized Design (CRD). Data collected for parameters related to early growth responses such as root, coleoptile and leaf length, root fresh and dry biomass, no. of tillers plant-1, and leaf total chlorophyll contents were subjected to R-package. Results showed significant differences among all early growth parameters genotypes in response to 5% and 10% PEG concentrations (p = 0.05). PEG concentration of 10% significantly reduced root length, root density, coleoptile length, root fresh and dry weight, and leaf total chlorophyll contents among all genotypes. Wheat landraces and advanced durum wheat lines showed better drought tolerance at 10% PEG concentration relative to two wheat cultivars AZRC Dera and Akbar-19 by maintaining optimal root length, coleoptile length, root density and root weight. Landrace acc#11239 and durum line IDN-733 were found to be most drought tolerant to 10% high PEG concentration in terms of root and coleoptile length and root fresh and dry weight. The findings of this study are important for breeders to evaluate wheat genotypes for drought stress tolerance and develop climate-resilient and drought-tolerant wheat cultivars for better yield production.
To evaluate the early growth response of six wheat genotypes (local cultivars, landraces, and durum wheat advanced lines) grown under Polyethylene Glycol (PEG)-mediated water stress conditions (5% and 10% PEG).
Six wheat genotypes were grown in Petri-dishes and pots under PEG-mediated water stress conditions (5% and 10% PEG) using a Completely Randomized Design (CRD) with three replications. Parameters measured included root length, coleoptile length, leaf length, root fresh and dry biomass, number of tillers plant-1, and leaf total chlorophyll contents. Data analysis was performed using the R-package for means and Least Significant Differences (LSDs).
graph TD; A[Select 6 Wheat Genotypes] --> B[Grow in Petri-dishes & Pots]; B --> C[Apply PEG Stress: 5% & 10%]; C --> D[Measure Early Growth Parameters]; D --> E[Statistical Analysis R-package]; E --> F[Identify Tolerant Genotypes]; F --> G[Conclusion on Optimal Screening Concentration]; G --> H[Recommendations for Breeding Programs];
Drought significantly impacts wheat yield, necessitating breeding programs for climate-resilient cultivars. Lab-based screening using PEG is crucial. Landraces and durum wheat lines demonstrated superior drought tolerance compared to modern cultivars (AZRC Dera and Akbar-19) in early growth parameters under PEG stress. Root length, root density, and coleoptile length are effective indicators for screening drought tolerance in the lab and field. The study suggests that 10% PEG concentration is an optimal level to induce significant lab-based drought stress in wheat.
PEG concentration of 10% significantly reduced root length, root density, coleoptile length, root fresh and dry weight, and leaf total chlorophyll contents across all genotypes compared to the 5% PEG treatment. Wheat landraces (specifically acc#11239) and advanced durum wheat lines (specifically IDN-733) showed better drought tolerance relative to cultivars AZRC Dera and Akbar-19 by maintaining optimal root length, coleoptile length, root density, and root weight under 10% PEG. Landrace acc#11239 and durum line IDN-733 were identified as the most drought tolerant to 10% PEG concentration.
The application of 10% PEG concentration is an optimal method for inducing lab-based drought stress to screen wheat genotypes. Landraces (acc#11239) and durum wheat lines (IDN-733) exhibited superior drought tolerance based on root length, root density, and coleoptile length, making them valuable for developing drought-tolerant wheat cultivars.
1. The study used six wheat genotypes comprising local wheat cultivars, landraces, and durum wheat advanced lines. (Confirmed in Abstract/Material section)
2. The PEG concentrations used to induce stress were 5% and 10% (w/v PEG in H2O). (Confirmed in Research Design section)
3. Landrace acc#11239 and durum line IDN-733 were found to be the most drought tolerant to 10% high PEG concentration. (Confirmed in Abstract/Results section)
Loading PDF...
Loading Statistics...