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ESTIMATION AND SEPARATION OF N-ACETYL-B-D-GLUCOSAMINIDASE ISOENZYMES IN URINE

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Title: ESTIMATION AND SEPARATION OF N-ACETYL-B-D-GLUCOSAMINIDASE ISOENZYMES IN URINE

Authors: Masood H. Javed , Abida N. Hussain , Mohammad A. Waqar 

Journal: Journal of Pakistan Medical Association

HEC Recognition History
Category From To
Y 2024-10-01 2025-12-31
X 2023-07-01 2024-09-30
X 2022-07-01 2023-06-30
X 2021-07-01 2022-06-30
X 2020-07-01 2021-06-30
W 2012-07-01 2020-06-30
X 2011-05-13 2012-06-30
Y 1900-01-01 2005-06-30

Publisher: Pakistan Medical Association.

Country: Pakistan

Year: 1992

Volume: 42

Issue: 3

Language: English

Categories
Urine Analysis (6) Enzyme Assays (3) Laboratory Medicine (487) Biochemistry (18816) Diagnostics (559)

Abstract

ABSTRACT


N-acetyl-B-D-glucosaminidase is a lysosomal enzyme made up of two isoenzymes (A and B). It has been used extensively as a marker for kidney damage. However, its estimation in urine has not been standardized. We have established a method for the estimation and separation of NAG isoenzymes by ion-exchange chromatography. In 19 experiments done so far, this method has given reproducible results. The significance of this method is that with a single experiment, one can estimate total as well as individual isoenzyme activity. Furthermore, urine constituents do not appear to interfere in this assay

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