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Title: COMPARISON OF ELISA, DIRECT ELECTRON MICROSCOPY AND ANTIBODY CAPTURE TECHNIQUE FOR DETECTION OF ROTAVIRUS IN HUMAN STOOLS - AN EXPERIENCE IN A DEVELOPING COUNTRY
Authors: Farrukh Azmi , Mubashir A. Khan , Saeed Tariq , Jamshaid Iqbal , Abdur Rab , Abdul Ghafoor
Journal: Journal of Pakistan Medical Association
Publisher: Pakistan Medical Association.
Country: Pakistan
Year: 1987
Volume: 37
Issue: 7
Language: English
Abstract
Two hundred and fourteen human faecal specimens were examined for detecting the presence of rotavirus by direct electron microscopy, antibody capture electron microscopy and ELISA. Twenty nine samples were positive with one of the three methods. Thirtyeight percent were positive with all three methods, 72.4% with ELISA, 62% with antibody capture electron microscopy and 55% with direct electron microscopy. ELISA was found to be the most sensitive method in our experience
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