DefinePK

Abstract

Abstract | Increasing material consumption and associated environmental concerns demand a shift from current fossils-based commercial manufacturing practices to sustainable, ecofriendly production systems. Extensive metabolic engineering practices incorporating living systems for commercial manufacturing of cost-effective value-added products and biochemical from sustainable feed-stock show limited success as desired higher production rates run counter to life-sustaining metabolic activities. To address these challenges, cell-free approaches developed as promising platform to proceed towards sustainable bio-manufacturing through the development of self-sustaining, constantly operating systems employing renewable biomass to yield higher concentrations and productivity of extended-range of products. To-date established cell-free systems including extract-based and purified enzyme-based cell-free technologies have been widely exploited for producing commercially valuable biopolymers and biochemicals such as building-block chemical, biofuels, bioplastic and value-added products. However, implementation of these cell-free systems at industrial production scale requires key considerations of sustainable carbon source, co-factors and energy regeneration pathways and biocatalyst engineering and recycling strategies to proceed towards sustainable production. At present, extensive research is required to address challenges of high cost associated co-factor supply and optimization of protocols to exploit full-potential of these cell-free technologies. Novelty Statement | This review article describe the potential utilization of cell free systems for the produc-tion of valuable chemicals through technological interventions which is the current demand of the society.