DefinePK

Abstract

Ophiophagus Hannah is extensively dispersed throughout various portions of the Asia. Many toxin proteins have been identified from their venom which are pharmacology active. Current research goals is to investigate the structural and function assessment of a hypothetical protein L345_13461 of Ophiophagus hannah for a better understanding of king cobra venom. Using in silico approach, the 3D structure was generated by Homology modelling, while the functions was profiled by ProFunc tool. The primary and secondary structure analysis revealed that the hypothetical protein L345_13461 is a stable and located in cytoplasm comprising of a remarkable number of random coils. Homology modelling was accomplished employing SWISS-MODEL server where the templates identity of 34.76% was observed with PDB ID 5ZZ3. Quite a few evaluations of quality assessment and validation parameters created a stable protein model with prodigious quality. Functional analysis was accomplished through InterProScan, ProFunc, DeepGoPlus and KEGG KAAG, suggesting that the hypothetical protein is a cytoplasmic protein, which plays important roles in protein binding, metabolism, signalling and cellular processes, genetic information processing. Finally, we proposed that experimental support would assist to investigate the structures and functions of other hypothetical proteins of various living organisms. 
Keywords: Function predication; hypothetical protein; Structure assessment; Ophiophagus hannah
http://dx.doi.org/10.19045/bspab.2021.100116