DefinePK

Abstract

The aim of this study was to develop an in-house high throughput method of crystallization and structure determination of lysozyme enzyme as model in structure-based drug design studies. Lysozyme stock solution at 50 mg mL-1 from hen egg white was crystallized using the vapour diffusion techniques in buffer A 0.1 M Na acetateacetic acid pH 4.8, 0.2 Na azide wv, 1.1 M NaCl and buffer B 0.1 M Na acetateacetic acid pH 4.8, 0.02 Na azide wv, 25 ethylene glycol wv, 1.7 m M NaCl. Two 24 well plates in duplicates were used for each buffer preparations and incubated at 4C. High quality crystals between 0.2-0.5 mm sizes, were produced in buffer A plate well after days 2 of incubation and none in buffer B. The crystal data collection and refinement showed a high resolution of the outer shell of 2.28-1.92 , completeness of 98.7, space group P43212 and mosaicity of 0.415.

PDF
References