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Title: Evaluation of physiological effects of examination stress on Quantitative red blood cells count and hemoglobin in female medical students at Peoples University of medical and health sciences for women, Nawabshah.
Authors: Reh Naz Sheikh, Seema Qazi, Hanna Khair Tunio, Sidra Jabeen, Nida, Iftkhar Ali Rathor, Masood Ahmed Unar
Journal: The Professional Medical Journal (TPMJ)
Publisher: Independent Medical College, Faisalabad- Pakistan
Country: Pakistan
Year: 2024
Volume: 31
Issue: 8
Language: English
DOI: 10.29309/TPMJ/2024.31.08.8106
Keywords: Red blood cellsExamination StressPhysiological Effects
Objective: To assess the physiological effects of examination stress on red blood cells in female medical students at Peoples university of medical and health sciences for women, Nawabshah. Study Design: Comparative Cross Sectional study. Setting: Department of Physiology, Peoples University of Medical & Health Sciences for Women, Shaheed Benazir Abad. Period: 7th April 2019 to 6th October 2019. Methods: A total 110 healthy students of first & Second year MBBS were included for this, by the non-probability randomized sampling technique, divided into two equal (55-students in each) groups A and B first & second year MBBS. Blood samples were collected prior to and during the exam, and the findings were evaluated using statistical software. Results: The mean RBC count ± SD thru the examination of students including the first year was 4.98±0.26 c/cm3, which was considerably higher than the pre-exam mean RBC count ±SD of 4.65±0.27 c/cm3. There was a small rise in RBC count throughout the examination compared to before (n = 55, p-value = 0.031) among the first-year students. During the second year examination, students had a mean RBC count ± SD of 4.67±0.28 c/cm3, which was not substantially higher than the pre-exam mean of 4.66±0.33 c/cm3. There was no significant increase in RBC count throughout the examination compared to before it among 2nd year students (n = 55, p-value = 0.932). Conclusion: Findings of study concluded that examination stress significantly affects first-year medical students' red blood cell (RBC) count, increasing it during exams, but not second-year students. This suggests varying physiological responses to stress, possibly due to academic adaptation or stress levels.
To assess the physiological effects of examination stress on red blood cells and hemoglobin levels in female medical students.
Comparative cross-sectional study conducted at the Department of Physiology, Peoples University of Medical & Health Sciences for Women, Shaheed Benazir Abad, from April 7, 2019, to October 6, 2019. 110 healthy first and second-year MBBS female students were divided into two groups of 55. Blood samples were collected before and during examinations. Data was analyzed using SPSS version 23, with Student's t-test for numerical variables.
graph TD;
A["Recruit 110 Female MBBS Students 1st & 2nd Year"] --> B["Divide into Two Groups"55 each""];
B --> C["Collect Blood Samples Before Exam"];
B --> D["Collect Blood Samples During Exam"];
C --> E["Analyze RBC Count and Hemoglobin"];
D --> E;
E --> F["Statistical Analysis SPSS v23, Student's t-test"];
F --> G["Interpret Results"];
G --> H["Draw Conclusions"];
Examination stress can cause fluctuations in hematological characteristics. The study suggests that first-year medical students experience a greater physiological response to examination stress, as evidenced by increased RBC count and decreased hemoglobin levels, compared to second-year students. This difference may be attributed to academic adaptation or varying stress levels. The findings for hemoglobin in second-year students were not statistically significant, which could be influenced by factors like menstruation.
- First-year students showed a statistically significant increase in mean RBC count during exams (4.98±0.26 c/cm³) compared to before exams (4.65±0.27 c/cm³), with a p-value of 0.031.
- Second-year students did not show a significant increase in RBC count during exams (4.67±0.28 c/cm³) compared to before exams (4.66±0.33 c/cm³), with a p-value of 0.932.
- First-year students showed a statistically significant decrease in mean Hemoglobin (Hb%) during exams (10.0204±1.67277%) compared to before exams (12.3484±1.66828%), with a p-value of 0.001.
- Second-year students did not show a significant decrease in Hemoglobin (Hb%) during exams (12.2600±1.47580%) compared to before exams (12.3927±1.54415%), with a p-value of 0.145.
Examination stress significantly affects the red blood cell count and hemoglobin levels of first-year female medical students, increasing RBCs and decreasing hemoglobin during exams. Second-year students did not exhibit significant changes in these parameters. This indicates varying physiological responses to stress based on academic year.
- The study included 110 healthy female students, divided into two groups of 55.
- The period of the study was from April 7, 2019, to October 6, 2019.
- For first-year students, the mean RBC count during exams was 4.98±0.26 c/cm³, which was significantly higher than the pre-exam mean of 4.65±0.27 c/cm³ (p=0.031).
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