DefinePK

Abstract

The varietal response of Feston and Nagina was tested using hypocotyl and leaf disc as
explant source. The callus formation was achieved on MS medium supplemented with 2,4-Dichlorophenoxy acetic acid 2,4-D and on MS medium supplemented with Napthalene
Acetic Acid NAA and Benzylamino Purine BAP from both explant sources. Shoot
formation from calli of Feston and Nagina were successfully obtained on different hormonal
levels of BAP NAA on MS medium. Maximums shoot percentages of calli derived from
hypocotyl 56.00 and leaf disc 42.30 was achieved on MS medium with 0.05mgl
IAA and 4.0mgl BAP for Feston. For Nagina, maximum shoot percentage was obtained from
leaf explant 39.13 and hypocotyl 32.00 on similar medium. Shoot regeneration
produced 3.137 and 2.353 mean number of shootsexplants for hypocotyl and leaf disc of
Feston whereas Nagina responded with 2.187 and 1.783 mean number of shootsexplant
for leaf disc and hypocotyl respectively.