DefinePK

Abstract

A series of experiments to explore genotypic behaviour of the two F1 tomato hybrids against
a multitude of hormonal regimes were conducted. The diverse explant sources like shoot
apices, nodal segments and root sections were subjected to MS media containing different
kinds and concentrations of plant growth regulators. Callus induction from cut surface of
the shoot tips was more on BAP-IAA enriched media than Kin-IAA enriched media in both
hybrids. Both hybrids regenerated 3-7 shoots per culture at Kin-IAA enriched MS media
whereas it was 2-3 shoots per culture at BAP-IAA enriched media. A minor callus induction
from nodal explants was observed in Kin-IAA enriched media in hybrid Bornia whereas
Royesta failed to induce callus. Bornia regenerated 1-2 shootsculture on Kin-IAA enriched
media where as Royesta induced 1 shootculture on both media tested. Both hybrids
produced non-embryogenic calli from root segments at 100 frequency on MS containing
IAA at all levels tested whereas they exhibited a low callus induction frequency on 2,4-D
enriched media. Clusters of long roots were found on IAA enriched MS media while no such
response was observed on 2,4-D enriched MS media.