DefinePK

Abstract

ABSTRACT


Background and Objective Breast tissue may experience structural alterations due to variables including infection, illness, inflammation, or exposure to deleterious compounds, with 7,12-Dimethylbenzaanthracene DMBA being a prevalent carcinogen utilized in cancer research. Zanthoxylum acanthopodium DC and Moringa oleifera leaves possess anti-inflammatory and anticancer effects. This study aimed to determine the expression of Her2, Cox-2 and Caspase 3 as markers of breast carcinoma after administration of Zanthoxylum acanthopodium DC and Moringa oleifera leaves. Materials and Methods This study used 36 rats with 6 groups, namely C0 Normal mice, C150 Only DMBA injection, C DMBA-injected ratsDoxorubicin, P1 DMBA-injected ratsZ. acanthopodium fruit, P2 DMBA-injected rats100 mgkg b.wt., of Moringa oleifera leaves and DMBA-injected rats50 mgkg b.wt., of Moringa oleifera leaves and 50 mgkg b.wt., of Z. acanthopodium fruit orally for 30 days then dissected and breast tissue was taken for immunohistochemistry procedures. The non-parametric data were analyzed using the Kruskal-Wallis and Mann-Whitney tests with a significance level of p0.05. Results The DMBA injection into the mammary tissue increases Her2 expression, infiltrates the surrounding tissue, including the stroma and forms a solid tumor mass with ambiguous boundaries. Zanthoxylum acanthopodium and Moringa oleifera alone reduced damaged tissue, however, their combined action boosted breast tissue Her2 expression but did not lower Cox-2 or Caspase 3 scores. The expression of Cox-2 in group C150 differed significantly from P1 p0.05, p 0.001. Conclusion Zanthoxylum acanthopodium and Moringa oleifera enhanced Caspase 3, which promotes apoptosis in breast cancer cells. Nevertheless, each plant individually diminished Her2 and Cox-2 expression more effectively than the combination.

PDF
Abstract
XML
References
Citation