DefinePK

Abstract

Blood samples were obtained from phenotypically normal Gaolao cattle breeds mainly young bulls. Out of 70 animals, one young bull aged one year exhibited inconsistent chromosomal number during routine cytogenetic investigation. The whole blood culture was set up in RPMI-1640 (HiMedia) medium for 72 hrs, harvested to prepare chromosomal slides for conventional G- banding using trypsin and Giemsa (GTG). A total 105 metaphases plates were screened under microscope and we observed inconsistent number of chromosome in 28 cells (26.6%) exhibiting 61,XY/59,XY/58,XY/57,XY. Mosaicism is usually associated with reduced fertility in male and infertility in females. The bull calf in present study was one year old; therefore its fertility could not be estimated. However, it is advisable that bulls having mosaicism should be checked for the fertility before being used for artificial insemination and breeding programmes.