DefinePK

Abstract

The serogroups B and I of Dichelobacter nodosus were selected as whole cell vaccine strains based on isolation and serogrouping studies in Chittoor district of Andhra Pradesh. The available serogroups B and I of D.nodosus were revived in TASH agar media with 2% hoof powder. The purity of antigens were checked and adjusted to a concentration of 1 × 108 mg/ml and 2×109 mg/ml. Later inactivated with Formalin and were adjuvanted separately using Montanide and Alum. Experimental trails were conducted in Sheep and Goat with two different adjuvants, namely Montanide and Alum and with the different antigenic concentrations i.e 1×109 organisms/ml and 2×109 organisms/ml. The immune response was high in vaccines prepared using antigenic concentration of 2×109 organisms/ml compared with antigenic concentration of 1×109 organisms/ml. Similarly among two adjuvants Montanide and Alum, Montanide adjuvanted vaccines were found to be better in eliciting immune response.