DefinePK

Abstract

Mycoplasmosis represents the most important group of infectious diseases in goats. There are several and well recognized species of Mycoplasma causing high morbidity and mortality to adult goats and kids. In the present study a total of 385 nasal swab samples of randomly selected goats were collected from Quetta abattoir for molecular detection of different Mycoplasma species. The DNA was extracted from the nasal swab samples by using genomic DNA purification kit (Gentra-Puregene, USA). The polymerase chain reaction (PCR) and restriction fragment length polymorphisms (RFLP) were performed for the molecular detection of different species of Mycoplasma. The highest prevalence for Mycoplasma mycoides cluster members 35(9%) was observed, followed by Mycoplasma mycoides sub-cluster members 29(7.5%) and Mycoplasma putrefaciens 19(5%) from all nasal swab samples (n=385) processed for the molecular detection of Mycoplasma species, while the lowest prevalence was detected for Mycoplasma capricolum sub sp.capricolum 6(6%). None of the DNA sample was found positive for Mycoplasma capricolum sub sp. capripneumoniae. The presence of Caprine Mycoplasma species detected by PCR seems promising as this technique is rapid, reproducible, and efficacious as compared to classical tools used in the diagnosis of Mycoplasma induced diseases in goats.