DefinePK

Abstract

Mastitis is one of the most common problems of dairy animals throughout the world. Mastitis causes heavy economic losses to the dairy industry worldwide. Many etiological agents are responsible for this disease. Staphylococcus aureus is one of the major causes for mastitis in cow and buffalo. Cow and buffalo mastitis is an inflammatory reaction in the udder and a main contagious disease. The aim of present research is to determine the biofilm forming ability and antibiotic resistance in Staphylococcus aureus strains isolated from clinical cases of cow and buffalo mastitis in Udaipur of Rajasthan, India. A total 182 clinical mastitis milk samples were examined for bacteriological studies, out of this total 40 (21.9%) S. aureus strains were isolated. Within total 40 S. aureus isolates 30 (75%) were displayed hemolytic activities on blood agar. For determination of biofilm forming ability 40 clinical isolates of S. aureus were investigated with Congo Red Agar (CRA) and Tube assay methods. Out of the 40 S. aureus isolates, 67.5% displayed biofilm positive by CRA method and 62.5% by Tube assay method. In CRA method, within 27 positive isolates, 10 (25%) showed high and 17 (42.5%) moderate biofilm producing ability were observed and in Tube method 8 (20%) showed high and 17 (42.5%) moderate strains. The results of Antibiotic susceptibility test revealed that biofilm forming S. aureus strains were 62.5, 60 and 45% resistance against ceftriaxone (30µg), ceftizoxime (30µg) and erythromycin (10µg) respectively, whereas non biofilm forming isolates were observed 10, 10 and 75% respectively. Present research work will be helpful in improve production of milk with quality and quantity, health and solve problems related to cow and buffalo mastitis and also increase annual income of dairy owners. Detection of biofilm forming ability in mastitis isolates may provide useful information for the establishment of a more adequate therapeutic treatment.