DefinePK

Abstract

The embryo production is carried out through a combination of techniques of collection of immature oocytes, in vitro maturation (IVM), fertilization (IVF) and culture (IVC). Samples collected from slaughterhouse are the cheapest and the most abundant source of primary oocytes for large scale production of embryos through in vitro maturation (IVM) and in vitro fertilization. The culture medium and selection of protein supplements and hormones for IVM play an important role in the subsequent maturation rate, and embryonic development following IVF. The in vitro fertilization procedures in buffalo and requires appropriate preparation of sperm and oocyte, as well as culture conditions that are favorable to the metabolic activity of the male and female gametes. The presumptive zygotes are then cultured in vitro up to the blastocyst stage at which these could either be transferred to synchronized recipients for producing live offspring or cryopreserved for future use.