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Abstract

Lactococcus garvieae is the causative agent of Lactococcosis in rainbow trout and it is introduced as a significant strain among Gram positive cocci. The scope of this study is detection of lactococcosis in rainbow trout by genotypic method and determination antibiotic susceptibility of Lactococcus strains. In this research, 100 rainbow trout were collected randomly from fisheries of Ayd?n province and its surroundings. The specimens were brought to Adnan Menderes University Faculty of Veterinary Medicine Department of Microbiology in cold chain. Samples taken from liver, spleen, kidney and heart were inoculated to Tryptic Soy agar and L. garvieae strains were identified by genotypic methods. Antibiotic susceptibility of L. garvieae strains were determined by disk diffusion method after PCR application. The antibiotics used in this research were Penicillin G, Florfenicol, Amoxycillin-Clavulanic acid, Ampicillin, Cefoperazone, Erythromycin, Methicillin, Gentamicin, Oxacillin and Cloxacillin. L. garvieae strains were detected susceptible to Amoxycillin-Clavulanic acid (90%), intermediate susceptible to Florfenicol (65%) and resistant to other remaining antibiotics in this study.