DefinePK

Abstract

Salmonellae are responsible for considerable losses in the poultry industry through the death of birds and loss in production especially Salmonella Enteritidis (SE). Two diagnostic tools were developed and compared to detect SE in poultry farms. Lateral flow immunochromatographic test (LFIT) was considered a field and laboratory test while PCR was absolutely laboratory test. The minimal bacterial count that gave positive LFIT and PCR was 102 CFU/0.1 ml and 10 CFU/ 0.1 ml respectively. For LFIT we could increase the sensitivity results to 1CFU/0.1ml by pre incubating the sample in trypticase soya broth at 37?C for 6 hour. About 100 samples were tested by the two developed methods and the results were compared with bacteriological methods. The sensitivity, specificity and accuracy of LFIT as compared to bacteriological examination were calculated and were found to be 91%, 80% and 90% respectively and for PCR was 98.8%, 82.3% and 96% respectively.