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Title: Detection of Quinolone Resistance through Amplification of the gyrA Gene of Mycobacterium species from Human and Animal Sources
Authors: Gemerlyn G Garcia, Ralph Kevin M Espinosa, Michelle A Miguel, Mark Lester Bernardino, Mark Arman D Aquino, Claro N Mingala
Journal: International Journal of Veterinary Science
| Category | From | To |
|---|---|---|
| Y | 2023-07-01 | 2024-09-30 |
Publisher: Unique Scientific Publishers
Country: Pakistan
Year: 2018
Volume: 7
Issue: 4
Language: English
Keywords: polymerase chain reactionMycobacterium avium aviumMycobacterium avium paratuberculosisMycobacterium tuberculosisQuinolone resistance
Ten (10) DNA samples of Mycobacterium tuberculosis (Mtb) isolated from sputum of TB-positive humans, DNA samples from Mycobacterium species isolated from lymph nodes and fecal samples of avians and bubaline animals were analysed by PCR targeting primers for gyrase A (gyrA), quinolone resitance A(qnrA) and topoisomerase IV (parC) genes. Results demonstrated that quinolone resistance recognized by gyrA was seen in one out of 10 DNA samples from human Mtb isolates and that no qnrA and parC genes were detected. The gene for quinolone resistance detected by the primer gyrA had a molecular weight of 333 bp. Resistance to quinolone mediated by gyrA, qnrA and parC genes in avian (M. avium avium) and bubaline (M. avium paratuberculosis) isolates of mycobacteria were not detected after PCR. The non-amplification of genes observed in this study explains the non-existence of quinolone resistance arbitrated by gyrA, qnrA and parC genes in the specified avian and bubaline mycobacterial isolates.
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