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Title: In vitro Maturation of Ovine Oocytes Using Follicle Stimulating Hormone (FSH), Estradiol-17?, and Co-Culture of Fallopian Tube Epithelial Cells (FTEC) in Tissue Culture Medium-199 (TCM-199)
Authors: Yon Soepri Ondho, Daud Samsudewa, Dela Ayu Lestari
Journal: International Journal of Veterinary Science
| Category | From | To |
|---|---|---|
| Y | 2023-07-01 | 2024-09-30 |
Publisher: Unique Scientific Publishers
Country: Pakistan
Year: 2020
Volume: 9
Issue: 1
Language: English
Keywords: MaturationMediumOocytePreantral folliclesOvine
This study was conducted to analyze the effect of FSH, estradiol-17ß, and co-culture of FTEC on ovine oocyte in vitro maturation. The material used was 2.290 oocytes taken from preantral follicles of 254 ovine ovaries. Study was carried out by testing four treatments: (T1) base medium (TCM-199, 5% FBS, NaHCO3), (T2) base medium + FSH, (T3) base medium + 17-? estradiol, and (T4) base medium + co-culture of FTEC. Result showed that percentage of mature oocyte (Metaphase II) in this study was 40.55%, 61.62%, 40.34% and 58.52% for T1, T2, T3, and T4, respectively. Statistical analysis showed that medium maturation treatments were highly significant different (P<0.01) on oocyte maturation (Metaphase II) percentage. The base medium containing FSH and base medium containing co-culture of FTEC are better medium than the base medium or medium supplemented with estradiol-17ß for ovine oocytes in vitro maturation.
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