DefinePK

Abstract

A combined inactivated montanide ISA-70 adjuvanted vaccine against two types of rabbit viral hemorrhagic disease (RVHD) and Pasteurella multocida (P. multocida) was prepared. Another 2 inactivated vaccines were prepared too, one bivalent RHDV1 and RHDV2 against RVHD while the other single monovalent against P. multocida. The prepared vaccines were compared from the aspect of immune response up on the vaccinated rabbits. The comparison was based on the estimation of the humoral immune response for both RHDV and P. multocida by hemagglutination inhibition test (HI) and indirect hemagglutination test (IHA). Four groups of susceptible rabbits were used; one for each vaccine and the 4th group was control. The immune response was followed up for 24 weeks. Higher and earlier immune response against both RHDV1and RHDV2 were induced by combined oil emulsified vaccines at 1st week post vaccination (WPV) than bivalent inactivated RHDV vaccine. Using HI, the antibody titer reached maximum levels of combined oil vaccine (211.75, 211.5) for RHDV1 and RHDV 2 at the 12th weeks after vaccination, respectively. The same results were for P. multocida vaccine as the higher and earlier immune response against P. multocida was obtained from the combined vaccine than Pasteurella vaccine only. For P. multocida A and D, at the 6th week after vaccination, antibody titers of combined oil vaccine using IHA reached (218.5,218.2) respectively. The challenge test in the combined vaccine group revealed that, RHDV1 gave protection with 90%, and 100% for RHDV2 in the combined vaccine. As well as challenge test for Pasteurella gave protection with 90%. The developed combined inactivated montanide oil vaccines against Rabbit hemorrhagic disease types 1, 2 and Pasteurellosis appeared to be safe and effective in improving the immune response (onset and longevity). Immune protection against RHDV1, RHDV2 and P. multocida can be achieved with one manipulation decreasing the stress on animals and efforts.