DefinePK

Abstract

Lactic acid bacteria (LAB) have the potential to be used as probiotics for broilers because they have the ability to release enzymes, one of those is cellulase enzyme. However, the lactic acid bacteria are still unknown; thus, they need to be found. This study aims to find cellulolytic LAB from soymilk waste as a probiotic candidate for broiler chickens. This research was conducted through several stages, those were isolation of LAB, LAB bacteria were then tested for their ability to produce cellulase enzyme. After that, the isolates were tested for their enzyme activity and the isolates obtained were then selected for their morphological characters. The results showed that the isolates found namely F4, F6, F9 and F11 were cellulolytic lactic acid bacteria. These four isolates are classified as gram-positive bacteria and are aerobic. The cellulase activities of isolates F4, F6, F9 and F11 were 17.69, 20.67, 14.72 and 13.13U/mL, respectively. Based on the characterization of the bacteria, isolates F4 and F6 were categorized as Lactobacillus sp.1, isolate F9 was marked as Lactobacillus sp.2 and isolate F11 was characterized as Lactobacillus sp.3.