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Abstract

The goal of this study was to detect Fusobacterium spp. in apparently healthy dromedary camels using qPCR and Microseq 500 sequencing. Six pregnant dromedary camels were swabbed for vaginal, rectal, nasal, and ocular discharges. Genomic DNA was extracted. qPCR followed by microseq 500 was used for the detection of Fusobacterium spp. The purity of the extracted DNA from various swabs ranged from 1.5 to 2.1. Detection of Fusobacteria using qPCR indicated their presence in 91.7% of the collected swabs. Fusobacterium spp. was found in all vaginal, ocular, and fecal swabs, but in 66.7% of nasal swabs. The Fusobacterium gene was abundant in vaginal swabs. The isolate was identified by 16S rRNA sequence as Fusobacterum gastrosuis CAMSA16 OQ824900. In conclusion, this study sheds light on some naturally occurring microbes in camels that, under specific circumstances, can cause serious diseases. F. gastrosuis may have zoonotic potential.