DefinePK

Abstract

Bovine viral diarrhea virus (BVDV) is an economically consequential animal pathogen that engenders substantial financial losses in the realm of cattle farming, and it maintains a prevalent presence on a global scale. Presently, the prevailing modality employed for its detection is enzyme-linked immunosorbent assay (ELISA), which necessitates the use of sophisticated instrumentation, protracts the duration of analysis, and is primarily suited for laboratory-based diagnostics. Regrettably, ELISAs do not lend themselves to convenient on-site detection within cattle farms. To surmount this predicament, colloidal gold particles were synthesized using tri-sodium citrate reduction, and subsequently harnessed to label the E2 protein (E2-Au). By amalgamating the gold standard binding release pad with colloidal E2-Au, we have devised a comprehensive assay system. The nitrocellulose membrane serves as the platform, whereby the detection line is adorned with E2-Au, while the quality control line is coated with proprietary polyclonal rabbit antibodies specific to E2. The method yields results within a span of 10-15 minutes, exhibiting an impressive total compliance rate of 93.36% (239/256) when compared to the commercial kit. The E2 test strip demonstrates specific reactivity with the anti-BVDV antibody, while avoiding any cross-reactivity with antibodies targeting Brucella, bovine foot and mouth disease, Mycobacterium bovis, bovine para-tuberculosis, bovine pasteurellosis, and bovine infectious rhinotracheitis. Consequently, the E2 strips offer heightened specificity, cost-effectiveness, and ease of operation, rendering them more convenient and expedient in comparison to ELISA kits. Utilizing the E2 strips, a comprehensive assessment encompassing 36 cattle farms and 2035 cattle in the vicinity of Xinjiang, China, was conducted. The positive rate within the group reached an impressive 91.67%, with individual positive rates standing at 54.05%.