DefinePK

Abstract

The text explores crucial technologies for safeguarding genetic resources, particularly local goats in Indonesia, emphasizing methods to protect oocytes essential for in vitro maturation and assisted reproductive technologies (ARTs) or stocks. Vitrification is identified as the optimal choice for preserving oocyte stocks. The study investigates the impact of heat shock on thawed goat oocytes, focusing on Malondialdehyde (MDA) and 8-hydroxy-2′-deoxyguanosine (8-OHdG) levels. Comparing a commercial cryoprotectant (P1) with ethylene glycol 30% and 1M sucrose (P2), the results reveal a significant difference in MDA levels between P1 (0.081±0.023) and P2 (0.421±0.053). Additionally, a trend suggests no statistically significant difference in 8-OHdG levels between commercial media (7614.08±250.56) and EG 30% + 1M sucrose (7815.02±191.91). In conclusion, the study underscores the complexities associated with cryoprotectant concentration, confirming that ethylene glycol 30% with 1M sucrose can effectively vitrify goat oocytes with careful exposure during warming and adherence to vitrification protocols.