DefinePK

Abstract

A preliminary study of total protein from the lactic acid bacteria (LAB) strain Pediococcus pentosaceus SR6 was proven to have an anticancer role in T47D cells. On the other hand, studies on the specific protein of this strain regarding its anticancer effect have so far never been revealed. Based on these considerations, this research was conducted. Research activities began by cultivating of LAB Pediococcus pentosaceus SR6 isolates, analyzing protein fragments using SDS-PAGE, isolating and electroeluting specific proteins from SDS-PAGE results, preparing MCF-7 breast cancer cell cultures, cytotoxic tests using the MTT cell proliferation and cytotoxicity kit method, and analyzing cell morphology via observation using the DAPI and Acridine Orange/Propidium Iodide (AO/PI) tests. The test results were then analyzed statistically using the SPSS25 program and then displayed in the form of Tables or Figures. The research results showed the 28.4kDa protein from Pediococcus pentosaceus SR6 was known to have anticancer activity with an IC50 of 5.42ppm. Their anticancer activity is selective because it is less toxic to normal cells which require a higher toxic dose, namely 28.36ppm. The protein has an ability to stimulate MCF-7 cells’ apoptosis and necrosis that is proved from the double staining with Acridine Orange/Propidium Iodide (AO/PI) and DAPI stainings. In conclusion, The 28.4kDa protein from LAB isolate Pediococcus pentosaceus SR6 was proven to be used as an breast anticancer compound with its selective ability against normal cells.