DefinePK

Abstract

Accurate identification of infectious molds from clinical specimens is crucial, as evidenced by the rising incidence of invasive fungal diseases in birds and their growing frequency. Fungal DNA was found in parrot feather samples linked to Psittacine Beak and Feather Disease (PBFD) virus infection following the detection of culture isolates. Internal transcribed spacer regions (ITS) have been employed as targets for phylogenetic research as they typically exhibit sequence variation between species, with modest change within strains of the same species. Numerous sequence variations were found when comparing ITS region sequences from reference and clinical isolates of six Aspergillus species, one Rhizopus arrhizus, and two Penicillium species, The addition of 5.8S rRNA gene sequences had little effect on the species as a whole due to the presence of little diversity between species in this region. Amplification, sequencing, and comparison with non-reference strain sequences in GenBank were performed on strains and clinical isolates of aspergilli and other fungi. Aspergillus and other species amplified ITS region had a size range of 540 to 608 base pairs (bp). A bootstrap of 1000 replications of every fungal isolate was also displayed on the phylogenetic tree, along with varying percentage rates among the fungal isolates.