DefinePK

Abstract

This study was conducted to obtain genetic markers in the PRL gene as one of the basic foundations of selection methods for the egg production performance of Sikumbang Jonti ducks. DNA extraction was performed using Promega’s Genomic DNA Purification Kit procedure. Amplification of the PRL gene was performed using the Polymerase Chain Reaction (PCR) method using a pair of forward and reverse primers for each fragment. Furthermore, the results of PRL gene amplification were electrophoresed using agarose media, which was as much as 1.5% with Ethidium Bromide staining. Finally, sequencing of PCR products of PRL gene fragments of Sikumbang Jonti ducks was carried out using the services of First BASE Laboratory in Singapore to be analyzed by direct sequencing using Dideoxy Sequencing ABI 3730 XL Automated DNA Sequencer. The results showed that there were six (6) mutations in the PRL gene at the position of intron 2 (g.1997 G>A, g.2090 C>A, g.2300 G>C), exon 4 (g.3778 T>A), and exon 5 (g.5929 C>A, g.5963 G>A) which were in Hardy-Weinberg equilibrium and associated with total egg production and length of egg stopping time. So, it can be concluded that the diversity of the PRL gene is used as a candidate genetic marker for egg production of Sikumbang Jonti ducks.