DefinePK

Abstract

This research aimed to determine the levels of 8-hydroxy 2-deoxy guanosine (8-OH-dG), Intact Plasma Membrane (IPM), Intact Acrosome Membrane (IAM), and motility of spermatozoa diluted with SemenLife® and Alpha Tocopherol. A completely randomized design was used with eight treatment groups, including semen diluted with SemenLife® diluent stored for 0 hours (control), 24 hours, 48 hours, and 72 hours, as well as the addition of 400 µg/mL alpha-tocopherol diluent with similar storage periods. After dilution, semen was stored in a styrofoam filled with icepack at an internal temperature range of 15-20oC. Observations were carried out on semen quality, which included 8OH-dG, IPM (%), IAM (%), and progressive motility (%), with each treatment repeated four times. The results showed that the addition of Alpha Tocopherol to SemenLife® diluent significantly (P<0.05) improved 8-OH-dG levels, IPM, IAM, and spermatozoa motility. In line with the length of storage, from 0 to 72 hours, there was a decrease in the quality of spermatozoa. Storage for 72 hours produced semen suitable for use with progressive motility of 42.50±1.29% and 48.50±1.29% for SemenLife® and SemenLife® diluent plus Alpha Tocopherol, respectively.