DefinePK

Abstract

The study aimed to identify the Immunoglobulin A (IgA) level fluctuations in colostrum during different lactation days, with practical implications for daily postpartum measurement. The purposive sampling of 15 lactating dairy cows’ colostrum was used in varied lactation periods (lactation 1-7) for 7 days with sampling time at 5 AM and 3 PM Indonesian Time. The IgA levels in colostrum from HF cows were measured using an ELISA kit assay following the standard protocol. The IgA levels in colostrum from cows at various lactation periods were analyzed using One-way ANOVA, followed by Duncan’s Multiple Range Test (DMRT). The highest IgA level (32.128±2.475mg/mL) collected at morning milking was in D3 and at afternoon milking was in D2 (32.444±5.098mg/mL). The lowest IgA level both at the morning and afternoon milking was in D1 with 26.583±1.774mg/mL and 27.687±2.10mg/mL, respectively. On average, the highest IgA level was in D3 at 30.958±2.582mg/mL, while the lowest average IgA level was in D1 at 27.135±1.941mg/mL. It was concluded that the mean IgA levels in colostrum increase from the first day (D1) to the second day (D2), then rise further, peaking on the third day (D3), before declining by the fourth day (D4). Subsequently, the mean values tend to stabilize with minor variations from the fifth day (D5) to the seventh day (D7).