DefinePK

Abstract

Brucellosis is one of the most dangerous infectious diseases of humans and animals. Therefore, monitoring the epidemiological situation in Kazakhstan and identifying sources of population infection make such studies a priority. The study aims to investigate brucellosis’s phenotypic and genotypic diversity, which caused the disease in the population and animals on the country’s territory. Serological methods were used in the study – immunoenzyme analysis using commercial test kit Ingezim Brucella Compac 2.0, microbiological methods – cultivation and microscopy of microorganisms isolated from pathological material, and polymerase chain reaction with multiplex procedure according to Bruce-Ladder method using commercial kit INgene Bruce-ladder V. As a result of the study, 48 animals with high antibody titers were selected from 990 animals, of which there were 413 cows and heifers, 552 goats and sheep and 25 animals of other farm animal species for sampling for microbiological culturing. As a result of culturing, microbiological culture growth was obtained in 28 samples. Microscopic examination of colonies on nutrient media and microorganisms did not reveal significant differences between pathogens from different disease foci. Polymerase chain reaction (PCR) typing revealed that in 70% of cases of the population, the source of the disease was Brucella melitensis, which was detected by PCR. In the other 30%, the causative agent was identified as type B. abortus, which was isolated mainly from the country’s northern regions. The studies that were conducted allowed us to clarify the circulation regions of different types of pathological brucellosis in Kazakhstan and to improve some methodological approaches in an epizootic survey of infection foci.