DefinePK

Abstract

In modern practical disinfection science, it is asserted that ideal chemical agents, besides having high antimicrobial activity and certain other properties, should also be environmentally safe, have minimal toxic effects on humans and animals, and be easy to dispose of after use. Our research aimed to evaluate the antimicrobial, cumulative, toxicological, and allergenic properties of anolyte, ozone, Shungite, propolis, and their complexes shozan, prozan, and shuprosan. To study the antimicrobial properties of the substances above and their complexes in vitro, we used the method of microbial culture growth inhibition in meat-peptone broth (MPB) and the disk diffusion method on meat-peptone agar (MPA) with test microbes – gram-positive cocci Staphylococcus aureus and gram-negative rods Escherichia coli. The results of the experiments were evaluated based on turbidity standards and the determination of the lysis zone. The toxicological properties of the substances were determined in white mice by intraperitoneal injection of the said substances. Our study’s results indicate that ozone, anolyte, Shungite infusion, and propolis infusion while demonstrating the antimicrobial activity of classical antiseptics, their complexes – shodan, frozen, and shuprosan – exhibit antimicrobial activity comparable to classical antiseptics such as ethanol and hydrogen peroxide against S. aureus and E. coli. Importantly, these complexes of natural origin antiseptics have been found to be safe, not showing toxicological, cumulative, or allergenic effects on laboratory animals, and meeting the required standards. This provides a strong basis for their potential use as antiseptic agents in medicine and veterinary practice.