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Abstract

This study aimed to determine the efficacy of the Aeromonas veronii bv veronii BmCL-03 vaccine to control the MAS disease of African catfish (Clarias gariepinus). The research used an experimental method with a completely randomized design (CRD), five treatments, and three replications. The treatments consisted of T1: intramuscular (i.m) injection; T2: intraperitoneal injection (i.p); T3: oral; T4: immersion; T5: without vaccination (control). Booster vaccination was carried out one week after using the same method, except for oral vaccination, which was given during the first ten days. In the third week, each fish was given 0.1mL of A. veronii bv veronii suspension at a 107 CFU/mL dose for all treatments as part of the challenge test. Antibody titer, survival rate (SR), relative percent survival (RPS), mean time to death (MTD), and growth rate are among the research factors. The data were analyzed using analysis of variance (ANOVA) and Duncan multiple range test (DMRT) at a test level of 5%. The results showed that the A. veronii bv veronii BmCL-03 vaccine was significantly different (P<0.05) and could increase antibody titer, SR, RPS, and weight gain of African catfish but was not significantly different (P>0.05) to fish length and MTD. Vaccination does not hurt the growth of African catfish. The vaccine of A. veronii bv veronii effectively protects African catfish, and the i.m injection treatment is the most effective. The A. veronii bv veronii vaccine has good prospects as a vaccine product that can improve the immune system and protect African catfish.