DefinePK

Abstract

Background:   Celiac disease (CD) is an inflammatory small intestine autoimmune disease.  The study aims to investigate the association and the detection of ATG5 polymorphism between celiac disease (CD) and   H. pylori  infection, and the association with Interleukin-33 (IL-33). Methods:  The study groups included patients from Iraq, ages 4 to 35. Two primary patient groups were created: sixteen had positive  H. pylori  and celiac disease, and thirty had positive CD and negative  H. pylori  disease. The levels of tissue transglutaminase IgA (TTG IgA),  H. pylori  IgG, and IL-33 were measured using the ELISA method. The primers were amplified using PCR. Results:  With celiac disease, the patient group's TTG IgA levels increased dramatically. The test also showed significant variations (P=0.054) in the  H. pylori  IgG levels between the patient and control groups. The  H. pylori  seropositivity test showed a statistically significant difference (p ≤0.033) between seropositive and seronegative individuals, while the patient group's IL-33 levels did not significantly differ (P ≤0.299) from the control group. Conclusions:  Our results showed that CD is more common in women and occurs in the age range of 24-35 years. It also showed that the mutant variant of ATG5 is associated with CD, and the significance of  H. pylori  IgG serum levels in the patient group may indicate that the bacteria involved in CD. Furthermore,  H. pylori  infection is more strongly linked to serum IL-33 levels than CD. Keywords: Celiac disease;  H. pylori ; PCR; IL-33; ATG5 polymorphism