DefinePK

Abstract

Background:   The World Health Organization estimates that 80% of the world's population uses plant extracts or their active components in traditional therapies. Recently, there has been a lot of interest in the quest for new antioxidant and antibacterial plant-based medications. Additionally,  Glycyrrhiza glabra  is a popular food flavoring and sweetening ingredient. Methods:  This work was to isolate and purify licorice root's glycyrrhizin acid (GA) and investigate its antimicrobial properties against  S. aureus  and  K.  pneumoniae  were isolated from urine , blood, wounds, ear water, sewage, soil, and water, totaling 12 isolates of each kind, Analytical method for the measurement of Glycyrrhizic acid  from the root extract that has been suitably supported by a mass spectroscopic analysis. Results : The result showed the effects of GA with different concentrations on  S. aureus  and.  K. pneumoniae . Obviously, the MIC range value was in concentration between 18.75 to 37.5 % for  S. aureus  isolates, and 4.68 to 75 for  K. pneumoniae  isolates. The result indicated that GA reduced the metabolic activity of cells in biofilm on  S. aureus  and  K. pneumoniae  with an inhibition percentage Conclusion : GA may have an effect on cell membrane permeability, biofilm formation, and efflux activity, which inhibits bacterial growth . Keywords: Glycyrrhizin acid;  Staphylococcus aureus ;  Klebsiella pneumoniae ; Antibiotic; Biofilm