DefinePK hosts the largest index of Pakistani journals, research articles, news headlines, and videos. It also offers chapter-level book search.
Title: Studies on the Efficacy of Heterogeneously Produced Pasteuria Penetrans (PP3) Isolate over Individual Pasteuria Isolates in the Spore Attachment, and Pathogenic Potential on Three Meloidogyne Species
Authors: Muhammad Shahid, Simon R. Gowen, Muhammad Burhan, Zeeshan Niaz, Anwar ul Haq
Journal: Plant Protection
Publisher: Center for Community Learning
Country: Pakistan
Year: 2023
Volume: 7
Issue: 1
Language: English
Keywords: BiocontrolRoot-knot nematodePasteuria penetransReproductive factorRoot galling
The aim of the present test was to develop a Pasteuria penetrans isolate ( Pp 3) on a Meloidogyne blend and compare the attachment and pathogenic potential of this heterogeneously produced isolate with other Pp 3 isolates produced on individual Meloidogyne species ( Meloidogyne javanica , M. incognita and M. arenaria ). Number of spores attached varied among Meloidogyne spp. Pp 3 isolate originally multiplied on M. javanica showed greater attachment level with second stage juveniles (J2s) of M. javanica (13.8) than other Meloidogyne spp. tested and lesser attachment was observed on J2s of a M. arenaria . The Pp 3 isolate showed variable influence on the Meloidogyne spp. in suppressing root-knot disease. There was a significant difference in numbers of eggmasses produced by females of Meloidogyne spp. Higher numbers of eggmasses were recorded with females of M. arenaria (370) while fewer numbers of eggmasses were observed in treatment where M. javanica (245) was present. Root galling differed significantly among treatments (P<0.01) and higher gall infestation was recorded in M. arenaria treatment (6.2) while lesser galling was observed in M. javanica treatment (4.4). Final female population also varied among treatments (P<0.05) showing higher numbers of females in M. arenaria treatment (456) and fewer with M. javanica (398). Parasitism of females of Meloidogyne spp. by Pp 3 differed significantly among treatments. Higher numbers of females infected with Pp 3 spores were recorded among females of M. javanica (14.4) while lesser numbers of parasitized females were observed in M. arenaria treatment (9.8). There were significant variations in numbers of endospores/female produced by females of Meloidogyne spp. however egg set data did not differ in the treatments. The results showed that the Pp 3 isolate was more pathogenic on M. javanica compared with other Meloidogyne spp. ( M. incognita , M. arenaria and M blend). The Pp 3 isolate was found compatible with different Meloidogyne spp. while M. arenaria proved the least good host to bacterial parasite
To develop a Pasteuria penetrans isolate (Pp3) on a Meloidogyne blend and compare its attachment and pathogenic potential with Pp3 isolates produced on individual Meloidogyne species.
The study involved multiplying Pasteuria penetrans isolate (Pp3) on a blend of three Meloidogyne species (M. javanica, M. incognita, and M. arenaria) and on each species individually. Spore attachment to second-stage juveniles (J2s) of Meloidogyne spp. was assessed. Pathogenicity was evaluated by inoculating tomato plants with encumbered J2s and measuring egg masses, root galling, final female population, and female infection by Pp3.
graph TD
A[Produce Pp3 isolate on Meloidogyne blend and individual species] --> B[Assess spore attachment to Meloidogyne J2s]
B --> C[Inoculate tomato plants with encumbered J2s]
C --> D[Measure egg masses, root galling, female population, and female infection]
D --> E[Analyze data and compare efficacy]
E --> F[Draw conclusions on pathogenicity and host suitability]
The study highlights variations in spore attachment and pathogenicity of Pasteuria penetrans isolates across different Meloidogyne species. M. arenaria appears to be a less suitable host for Pp3. The heterogeneously produced Pp3 isolate (from a blend) demonstrated improved spore attachment and pathogenicity, suggesting that a broader genetic base in the bacterial parasite can enhance its effectiveness against diverse nematode populations.
- The Pp3 isolate multiplied on M. javanica showed greater spore attachment to M. javanica J2s (13.8) compared to other Meloidogyne spp., with the least attachment on M. arenaria J2s (7.73).
- The Pp3 isolate showed variable suppression of root-knot disease across Meloidogyne spp.
- M. arenaria exhibited higher numbers of egg masses (370) and root galling (6.2) compared to M. javanica (245 egg masses, 4.4 galling).
- M. javanica showed higher infection rates by Pp3 (14.4%) compared to M. arenaria (9.8%).
- The Pp3 isolate multiplied on the Meloidogyne blend was found to be more pathogenic on M. javanica than on other Meloidogyne spp.
The Pp3 isolate was more pathogenic on M. javanica compared to M. incognita, M. arenaria, and the Meloidogyne blend. M. arenaria was the least suitable host for the bacterial parasite. A heterogeneously produced Pp3 isolate, cultured on a blend of Meloidogyne species, showed greater potential for biocontrol compared to isolates cultured on single species.
- The study was published in Plant Protection, Volume 07, Issue 01, in 2023.
- Spore attachment was significantly different among Meloidogyne spp. (P<0.01).
- Root galling differed significantly among treatments (P<0.01).
Loading PDF...
Loading Statistics...