DefinePK hosts the largest index of Pakistani journals, research articles, news headlines, and videos. It also offers chapter-level book search.
Title: Surveillance of Bacterial Canker of Peach in Azad Jammu and Kashmir, and its Bio-Management
Authors: Basharat Mehmood, Muhammad Saeed, Asif Ur Rehman, Saqib Saleem, Muhammad Riaz Khan, Muhammad Tahir Younas, Muhammad Shafqat, Zahid Hussain Khan, Shafiq-ur- Rehman, Sajjad Hussain
Journal: Plant Protection
Publisher: Center for Community Learning
Country: Pakistan
Year: 2023
Volume: 7
Issue: 1
Language: English
Keywords: IncidencePseudomonas SyringaeBacterial diseasesDisease prevalence
Bacterial canker of peach caused by Pseudomonas syringae is an increasing problem in Azad Jammu & Kashmir with more than 50 pathovars on the basis of host. A detailed survey was conducted in peach growing areas of Azad Jammu and Kashmir for the determination of disease prevalence and incidence. Pathogen was isolated and characterized from the collected samples using LOPAT and GaTTA scheme. Furthermore the results of biochemical identification was confirmed using PCR followed by sequencing of obtained expected bands. For the bio-management of virulent pathogenic strains indigenous rhizobacterial isolates were obtained, characterized and evaluated using dual culture as well as in a greenhouse pot experiment against P. syringae pathovars. During a positive sampling method used for the surveillance it was found that maximum disease prevalence was 75% in district Bagh while there was 25% disease prevalence found in Haveli and Sudhanoti. Similarly maximum disease incidence was found in district Bagh that was 16%. A total of 32 isolates were recovered from the collected samples that were further screened on the basis of their virulence. It was found that the total of 18 isolates screened were similar to the already reported strains of P. syringae pv. syringae (Pss) on the basis of biochemical and molecular tools. From total 17 rhizobacterial isolates recovered were subjected to dual culture technique and was found that 06 isolates showed promising zone of inhibition upto 11.5 mm. The results of greenhouse evaluation revealed that the minimum disease incidence was recorded 3% using R-9 and R-17 in consortium against Pss as compared to the control treatment (39%). It was concluded that the ecofriendly management practices against this destructive disease must be adopted and appreciated
To survey and determine the prevalence and incidence of bacterial canker of peach in Azad Jammu & Kashmir, isolate and characterize the pathogen, and evaluate indigenous rhizobacterial isolates for bio-management of the disease.
A detailed survey was conducted in peach-growing areas of Azad Jammu & Kashmir to assess disease prevalence and incidence. The pathogen was isolated and characterized using LOPAT and GAATa schemes, with confirmation via PCR and sequencing. Indigenous rhizobacterial isolates were obtained, characterized, and evaluated for bio-management using dual culture and greenhouse pot experiments against P. syringae pathovars.
graph TD
A[Survey of Peach Growing Areas] --> B[Sample Collection];
B --> C[Isolation of Pathogen];
C --> D[Characterization: LOPAT, GAATa, PCR];
C --> E[Isolation of Rhizobacteria];
E --> F[Characterization of Rhizobacteria];
F --> G[In Vitro EvaluationDual Culture];
G --> H[Greenhouse Evaluation];
D --> I[Pathogen Identification];
H --> J[Data Analysis];
J --> K[Conclusion and Recommendations];
The study highlights the significant prevalence and incidence of bacterial canker in Azad Jammu & Kashmir, caused by Pseudomonas syringae. The isolation and characterization of the pathogen using both biochemical and molecular tools confirmed its identity. The evaluation of indigenous rhizobacteria demonstrated their potential as biocontrol agents, with some isolates showing significant inhibition of the pathogen in vitro and disease suppression in vivo. The findings support the adoption of ecofriendly management practices.
Maximum disease prevalence was 75% in district Bagh, while disease incidence was 16% in the same district. A total of 32 P. syringae isolates were recovered, with 18 confirmed as P. syringae pv. syringae (Pss). Six rhizobacterial isolates showed promising zones of inhibition (up to 11.5 mm) in dual culture. In greenhouse experiments, minimum disease incidence was 3% using R-9 and R-17 in consortium against Pss, compared to 39% in the control.
Bacterial canker, caused by P. syringae pv. syringae and morsprunorum, is prevalent in Azad Jammu & Kashmir. Ecofriendly management practices, including the use of biocontrol agents and quarantine measures, are recommended. The research findings can aid in breeding programs for resistant peach varieties.
* Maximum disease prevalence was found to be 75% in district Bagh.
* Maximum disease incidence was recorded as 16% in district Bagh.
* Six rhizobacterial isolates showed promising zones of inhibition up to 11.5 mm in dual culture.
Loading PDF...
Loading Statistics...