DefinePK

Abstract

In this study, optimization of Averrhoa bilimbi and Citrus hystrix extraction using maceration extraction methods was performed using a statistical method design of experiment (DOE). A two factorial design of experiment was performed with ethanol concentration and ratio of solid to liquid as the controlled variables. Results showed that an optimized maceration extraction method of phenolic compounds from Averrhoa bilimbi and Citrus hystrix peel have been developed the highest TPC value of Averrhoa bilimbi crude extract, i.e. 5.45 mg GAE/g Averrhoa bilimbi powder was obtained at the following maceration condition: ratio solid to liquid of 1 to 15 using ethanol with concentration of 48% as solvent. Whilst, for citrus peel crude extract, the highest TPC value was 55.71 mg GAE/g dried citrus peel under the following maceration condition: ratio solid to liquid of 1 to 50 using aquades as solvent. From DOE analysis, it is known that controlled variables used have significant effects to TPC as response, even though there was no significance interaction between variables.