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Abstract

This paper present study of single molecule Human Papillomavirus (HPV) DNA through the gold nanoparticle system by using atomic force microscopy. Transduction of biorecognition events into electrical signals through the integration of single biomolecule HPV DNA probe in bioelectronic nanodevices requires both a reliable electrical contact and the metallic electrode for efficient conduction mechanism. These conditions have been met in the hybrid system obtained by linking gold nanoparticles with DNA probe. Such an assembling strategy, combined with a conductive atomic force microscopy (AFM) investigation, has allowed us to put into evidence an unprecedented matching between current and topography features and to attribute the intramolecular charge transport. Nanoparticles have been explored as signalling probes for Ultrasensitive DNA detection that can be used in field applications to overcome these limitations. Gold nanoparticles (AuNPs) have been extensively used mainly because of its optical property and ability to get functionalized with a variety of biomolecules. The study demonstrates the use of gold nanoparticles functionalized with single stranded oligonucleotide (GNP- oligo probe) as visual detection probes for rapid and specific detection of Human Papillomavirus (HPV).