DefinePK

Abstract

Rapid identification of viruses has been important implications for medical healthcare. This paper presents an analysis study of a single gold nanoparticle system of Interdigitated Device Electrodes (IDEs) by using Energy-Dispersive X-ray (EDX). The presently available methods of detection are mainly based on rapid immunoassay techniques that require trained manpower and are time consuming and expensive. However a biosensor facilitates a doctor to identify the specific virus quickly and prescribe an appropriate course of treatment. In this study, a nanobiosensor based on gold nanoparticles (GNPs) and oligonucleotide probe was designed for the visual detection of Human Papillomavirus (HPV). A simple and facile method for the depositing of gold nanoparticles (GNPs) colloid was described via a simple deposition method on Interdigitated device electrodes (IDEs). The GNP pattern deposition on IDE was investigated by using an SEM combined with an EDX. The study demonstrates the use 30 nm of gold nanoparticles functionalized with single stranded oligonucleotide (GNP- oligo probe) as visual detection probes for rapid and specific detection of HPV. By addition of gold nanoparticles, it enhances the signal and sensitivity of IDE for detection of HPV.