DefinePK

Abstract

The commercialization of biodiesel and their diesel blends on the market of many countries would result towards environmental damages due to accidental spills such as leakage. This study aims to investigate the survival of Pseudomonas putida as bioremediation agent in soil contaminated with biodiesel/diesel blends (B50). The spillage stimulation of B50 was conducted at laboratory scale for 24 days of treatment time. The experimental results show that introduction of biodiesel/ diesel blends into the soil induced a reduction in growth of Pseudomonas putida in relation to control values. The Pseudomonas putida growth was influenced by with or without biodiesel/diesel content. The growth of Pseudomonas putida gained from contaminated soil was higher in control sample followed by B50. These findings enhance our understanding that Pseudomonas putida is sensitive to any ecosystem perturbation, and measurement of their colonies may serves as indicators of soil pollution and soil health.