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Abstract

In this study, the performance of HNO3 and NaOHpretreatmentson oil palm mesocarp fiber (OPMF) was evaluated based on the residual carbohydrate. The best pretreatment condition was ascertained for reducing sugar and biosugar produced after saccharafication with viscozyme. The pretreatment with 2 % (v/v) HNO3 improved cellulose concentration from 33.14 % to 60.0 %, while hemicellulose, lignin and ash were reduced by 25.59 %, 10.0 % and 25.9 % to 10.0 %, 5.33 % and 3.0 %, respectively. Enzymatic treatment using 1g solid loading, produce highest production in terms of arabinose 10.5 g/l, glucose (1.1 g/L) and xylose (0.42 g/L) was obtained under agitation. Whilefor 10 grams solid loading, highest level of bio-sugar was obtained with arabinose (0.8 g/L) and glucose (1.6 g/L). FESEM-EDX and FTIR images before and after saccharification successfully showed the effect of enzymatic treatment on the mesocarp fiber.