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Abstract

In this investigation nano silver was prepared by reduction of silver nitrate using fenugreek extract. Use of natural reducing agent in the synthesis of nano silver is an environmentally friendly process in contrary of using chemical reducing agent. High resonance transmission electron microscope (HR-TEM) and X-ray diffraction (XRD) tests are used to characterize prepared nano silver. The results confirmed that, the morphology of the prepared nano silver were of spherical shape with smooth surface and average diameter of 17nm. Produced nano silver was tested as antibacterial agent and it is successful against E-coli and staphylococcus aureus due to the increase of the inhibition zone than using 1 molar silver nitrate alone i.e. the use of nano silver increased the inhibition zone of E-coli and staphylococcus aureus. So the synthesis of nano silver leads towards chemical use as antibacterial agent.