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Abstract

Objectives: To evaluate the preventive role of ZincChloride on Toxicity of Ciprofloxacin administration on secondary ossification centers in wistar albino rat litters.Design of Study: Prospective and comparative animal study carried out on experimental Wistar Albino Rats.Setting: The study was carried out in the department of Anatomy Basic Medical Sciences Institute, JinnahPostgraduate Medical Center (JPMC) Karachi from 2001 to 2002. Methods: Ciprofloxacin and Zinc Chloridewere administered to newly born albino rat pups separately and simultaneously at a dose of 20 mg/kg bodyweight and 120 ug/100g body weight respectively intraperitoneally twice daily from day 1 to 14 after birth.These animals were killed by deep either anaesthesia and fixed in 80% alcohol. They were then bulk stained withalizarin Red S and alcian blue. Finally they were cleared in 4% KOH and stored in Glycerin. The fore and hindlimbs were disarticulated from the axial skeleton and observed under stereo-microscope for evidence of skeletaldifferentiation in the form of presence of secondary ossification centers in ling bones. The time of appearanceof these centers were noted and compared statistically with those in the control animals. Results: This studyrevealed that the secondary ossification centers in long bones were delayed in experimental animals andsimultaneous treatment with Zinc Chloride partially prevented toxicity of ciprofloxacin as compared withcontrols. Conclusion: these results strongly suggest that ciprofloxacin causes a risk to skeletal differentiation and therefore to its growth. However the cirpofloxacin toxicity could ne partially prevented by simultaneousadministration of Zinc Chloride in wistar albino rat litters.