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Abstract

Objective: This study was aimed to assess the histomorphological changes in rat mandible induced by injectionbotox in masseter muscle and to assess the possible remedial effects of grape seed proanthocyanidin extract(GSPE) on histomorphology of the rat mandibular condylar cartilage.Study Design: Laboratory based experimental study.Place and Duration of Study: This study was conducted at the Department of Anatomy and Pathology, ArmyMedical College Rawalpindi, Pakistan from 16th June 2020 to 07th August 2020, with the collaboration of theMilitary Hospital, Rawalpindi and the National Institute of Health (NIH), Islamabad.Methods: Forty, healthy, female (non-pregnant) “Sprague Dawley rats”, 10 weeks of age with weight of 200-250gm were selected and divided into four groups. Group A control, Groups B, C and D were experimentalgroups. Group B was sham injected whilst group C and D were injected by botox in left masseter muscle. GroupD was treated with Grape seed proanthocyanidin extract for 1 month whereas rats from group B and C weregiven saline solution as a vehicle. H&E and Toluidine Blue after decalcification of the mandible were used forstaining. Data was analyzed using the SPSS 23. The P-value of ≤0.05 was considered significant. One-wayanalysis of variance was applied followed by Tukey's post-Hoc test to seek distinction of quantitative variablesbetween the groups.Result: Botox injected group C developed changes when compared with the control group A and sham group B,including the discontinuation of the condylar cartilage surface and chondrocyte hypertrophy, cluster formationand disorientation of chondron columns. The experiment did not show any improvement in experimentalgroup D after treatment with grape seed proanthocyanidin extract.Conclusion: The administration of grape seed proanthocyanidin extract did not yield the expectedimprovements in the histomorphology of the mandibular condylar cartilage.
How to cite this: Iram M, Kiani MRB, Qamar A, Atta MF, Shan M, Zia MS. Ameliorative Effects of Grape Seed Proanthocyanidin Extract on Histomorphological Changes in Rat Mandible Induced by Intramuscular Injection of Botox in Masseter. Life and Science. 2024; 5(4): 482-490. doi: http://doi.org/10.37185/LnS.1.1.766