DefinePK

Abstract

Objective: This study aimed to explore the protective effects of vitamin C against Methotrexate inducedbiochemical and histological changes in rats.Study Design: Experimental study.Place and Duration of Study: The study was conducted at the Department of Anatomy, Baqai MedicalUniversity Karachi, Pakistan from June 2017 to July 2018.Methods: This study included 45 adult male albino Wistar rats aged between 10-12 weeks and weighing around180-200g. The rats were randomly divided into 3 groups: Group A, received no intervention and was kept onstandard diet for 10 days; Group B, received 20mg/kg intraperitoneal single dose of Methotrexate; Group Creceived 200 mg/kg oral tablets of vitamin C for seven days and 20mg/kg intraperitoneal single dose ofMethotrexate. At the end of the study period, animals were anesthetized with ether, cardiac blood sampleswere collected for enzyme estimation and organs were removed for histopathological examination.Results: Liver sections from Group A demonstrated normal parenchyma with cords of hepatocytes radiatingfrom the central vein with portal triads at the ends of hepatic lobules while Methotrexate treated Group Bshowed severely degenerated architecture along with hemorrhagic parenchyma with dilatation of central vein,and sinusoids. Comparatively, liver sections from Group C displayed less deranged histologic and morphologicchanges. A significant increase in serum levels of AST, ALT and ALP and albumin and reduction in hepatocytecount, increase in hepatocyte diameter and a decrease in hepatocyte nuclear diameter occurred in group B,while above parameters improved in vitamin C protected group C.Conclusion: This study exhibited that the potential biochemical and histological alterations caused byMethotrexate can be attenuated by antioxidant effects of Vitamin C.
How to cite this: Younus N, Kazmi T, Askari R, Ali AB, Rasheed A, Mukhtar S, Nangrejo R. Antioxidative Effects of Vitamin C on Methotrexate Induced Hepatic Damage in Rat. Life and Science. 2024; 5(4): 521-529. doi: http://doi.org/10.37185/LnS.1.1.626