DefinePK

Abstract

Objectives: To evaluate the in vitro antibacterial effect of three different restorative materials (Glass Ionomer Cements (GIC)) containing chlorohexidine on Streptococcus mutans, and lactobacillus acidophilus.
Materials and Methods: Three commercially available glass ionomer cements, i.e., Fuji 1X (GIC1), Ketac molar (GIC2) and Riva (GIC3) were evaluated each alone and in combination with chlorohexidine diacetate or chlorohexidine digluconate. GICs were manipulated in accordance with manufacturer’s guidelines and embedded in wells made-up in plates of trypticase soy agar seeded with Streptococcus mutans and Lactobacillus MRS agar seeded with Lactobacillus acidophilus. The antibacterial activity was evaluated by using a caliper to measure the diameter of growth inhibition zones. The study was performed in triplicate and Duncan post-Hoc Multiple comparisons at p≤ 0.05 is used for means comparison.
Results: the three Glass ionomers with chlorohexidine diacetate powder (1%) showed the highest activity and prolonged effect on the tested strains compared to glass ionomers free from chlorohexidine and the other glass ionomers with chlorohexidine digluconate liquid. Also, it was found that Fuji IX glass ionomer showed higher and prolonged effect in comparison to Ketac-Molar and Riva glass ionomers. Glass ionomers in combination with chlorohexidine diacetate showed higher efficacy against streptococcus mutans than for lactobacillus acidophilus.
Conclusion: All three GIC’s under evaluation, promoted growth inhibition of the cariogenic bacteria assayed. Fuji IX glass ionomer with chlorohexidine diacetate showed the highest efficacy and durability against the tested strains.