DefinePK

Abstract

Background:   The presence of microorganisms in milk cannot be totally prevented, making it a crucial factor in determining its quality.  Staphylococcus aureus  is one of the germs that can be detected in milk and other dairy products. Many  S. aureus  related foodborne disease outbreaks have been reported to be caused by multidrug-resistant (MDR) strains. Methods:  In the Indonesian province of East Java, a total of 112 raw milk samples were taken from various dairy farms.  10 ml of milk was collected from each cow using a sterile bottle. The Milk samples were transported to the laboratory under a cold chain and analyzed using standard microbiological procedures. Evaluation of  S. aureus  isolates for antibiotic susceptibility using the Kirby-Bauer disc diffusion method. Results:  Findings from this study showed that 100 (89.2%) out of 112 samples of raw milk taken from dairy farms in 2 regions located in Pasuruan and Lumajang yielded positive for  S. aureus . The antibiotic sensitivity profile showed that Cefoxitin was the most effective with 2% resistance. The highest resistance recorded was against Ampicillin (78%). Resistance recorded against other antibiotics include Tetracycline (36%), Erythromycin (11%), and Amoxicillin (12%). Multidrug resistance was recorded for  S. aureus  isolates from 10 samples of raw milk. Conclusion:  The study's conclusions highlight the risk of milk contamination and the potential for  S. aureus  to develop multidrug resistance, both of which are harmful to human health. It is strongly recommended that strict hygiene practices be maintained and improved in dairy farms. Keywords:    Staphylococcus aureus ; Multidrug resistance; Raw milk; Human health; Indonesia