DefinePK

Abstract

The current study was conducted at University of Kufa, Faculty of Science, Biology Department for molecular identification of four Eruca sativa L. cultivars (1-Turish 2-Syrian 3-Turkish 4-Eygyptian) using eleven Random Amplified Polymorphic DNA. Results indicate primers OPA-04, OPA-10, OPA-02, OPW-04, OPC-09,v and OPD-13 success in giving all cultivars a unique fingerprint .Highest number of   main bands , polymorphic bands and discriminatory value were 20 , 18 and 20.22% respectively produced by OPA-02. Highest value for amplified band number and monomorphic bands were 44 and band produced by primers OPX-17 and OPD-13 respectively.  Highest value for both polymorphism and Efficiency were 94.11% and 0.48 respectively produced by primer OPA-04. Genetic relationship and genetic distance among E.sativa cultivars distribution unrelated to their geographical origin. RAPD markers are efficient tool in studying E.sativa germplasm.