DefinePK

Abstract

In this work, a new device is designed for measuring the light intensity of the Bioluminescence reaction between Luminol with Hydrogen peroxide that is produced as an activity of Triglycerides Phosphate Oxidase. The project includes the use of a homemade and semi-automated device for the determination of Chemiluminescence (CL) and Bioluminescence light by direct reaction analysis of Luminol with oxidant and using a Mobile –phone as a recorder which is employed in chemistry with Arduino and applied this device into the enzymatic reaction for determine Triglyceride by this new methods. The method is easy, simple, and rapid with high sensitivity for the determination of hydrogen peroxide. The light of the Bioluminescence reaction of Luminol (5-amino-2,3-dihydro-1,4_phthalazinedione) with oxidant (H2O2) was sensed by photocell and the signal was sent to Mobile. The method used to determine glycerol and compare the results of the device with real value to give high accuracy, Triglyceride was determined in its kit. It gave 99.2 and ±0.1 of Recovery and RSD, respectively.